Coding

Part:BBa_K2207023:Design

Designed by: Zifan Xie   Group: iGEM17_ZJU-China   (2017-10-21)


Serine protases comes from P.lilacinum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When our T.atroviride is activated by the signals which have been described in our wiki, they will produce serine protein to hydrolyze the nematode eggshell, so that the root knot nematodes cannot grow normally.


Source

It comes from Purpureocillium lilacinum.

References

[1]Wang J P, Wang J X, Liu F, et al. Enhancing the virulence of Paecilomyces lilacinus against Meloidogyne incognita eggs by overexpression of a serine protease. Biotechnology Letters, 32, 1159-1166[J]. Biotechnology Letters, 2010, 32(8):1159-1166.

[2]Brand D, Roussos S, Pandey A, et al. Development of a bionematicide with Paecilomyces lilacinus to control Meloidogyne incognita.[J]. Applied Biochemistry & Biotechnology, 2004, 118(1-3):81-88.